IgA肾病患者肾组织内synaptopodin的表达及意义

Significance of synaptopodin protein expression in renal tissues in patients with IgA nephropathy

目的观察synaptopodin在IgA肾病患者肾组织中的表达,并探讨其在肾组织分布特征、进行相关分析,推测病情预后。方法分析202例IgA肾病患者的肾活检组织标本,按Lee分级系统分为5级及参照Katafuchi积分方法,依据病变程度进行积分。对选取的样本经抗人synaptopodin单克隆抗体间接免疫荧光染色。用免疫荧光显微镜观察及图像分析软件进行定量分析肾组织中synaptopodin的表达情况,测量平均光密度值,然后进行统计学分析。结果对5组的IgA肾病患者肾组织中synap-topodin所表达的平均光密度值进行比较,差异有统计学意义(P<0.01),病理组织中synaptopodin所表达的平均光密度值与Katafuchi积分存在负相关关系(r=-0.922,P<0.01)。结论 synaptopodin可做为判断肾纤维化程度及预后的重要指标之一。

Objective To investigate the expression of synaptopotin protein in renal tissue in patients with IgA nephropathy,discusses the distribution characteristics of synaptopodin in renal tissues,And putative prognosis after correlation analysis.Methods The study including 202 inpatients with IgA nephropathy,all the cases were divided into five grades according to the Lee′s classification,referred to Katafuchi scoring system,and calculated pathological integrals according to the pathologic change degree in the renal tissue.Subsequently,the renal biopsy specimens were treated with mouse anti-IgG synaptopodin by indirect immunofluorescent stain.Expression intensity of synaptopodin was observed by fluorescence microscope and imagepro plus was applied to quantity the synaptopadin expression,measured the glomerlular IOD SUM and area SUM and then the statistical analysis.Results The mean density of synaptopodin expression had a significantly different among the five groups of renal tissues in patients with IgA nephropathy（P〈0.01）,katafuchi scoring system of renal pathological change were gradually increased from grade I to IV.Conclusion Synaptopodin may be an important factor for predicting renal fibrosis and prognosis of IgA nephropathy.