HPLC法测定克痨星胶囊中黄芪甲甙的含量

Determination of astragaloside Ⅳ in Kelao Xing Capsules by HPLC

目的 :为克痨星胶囊中黄芪的含量测定建立质控标准。方法 :将黄芪中的环黄芪醇类皂甙用 1%的醇制氢氧化钾皂化 ,使糖部分的乙酰基均转变为羟基 ,使环黄芪醇类皂甙均转变为黄芪甲甙 ,以HPLC方法进行检测。HPLC条件 :Waters公司NovapakC18( 3.9mm× 15 0mm)色谱柱 ,40 %甲醇为流动相 ,检测波长 2 0 4nm。结果 :本方法线性范围 0 .72～ 1.6 2 μg ,回归方程Y =1.72 47× 10 -5X +1.5 34× 10 -6,r =0 .9989,平均回收率 94.45 % ,RSD为 2 .0 3% (n =6 )。结论

Aim To determine the astragaloside Ⅳ in Kelao Xing Capsules.Methods Cyclo astragenol in astragalus membranaceus was saponified by 1% potassium hydroxide in alchol and the acetyl group in xylose was changed to hydroxy group.i.e cyclo astragenol was changed to astragalosied Ⅳ and was detected by HPLC.HPLC conditions:Waters Novapak C 18 (3.9mm×150mm) column;40% methanol as mobile phase and detected at 204nm.Results The linear range is 0.72～1.62μg(Y=1.7247×10 -5 X+1.534×10 -6 ,r=0.998 9).The average recovery is 94.45%,RSD=2.03%(n=6).Conclusion Using the method can determine the astragaloside Ⅳ in Kelao Xing Capsules quickly and exactly.