6种栽培品种南瓜提取物的α-葡萄糖苷酶抑制活性的研究

Study on α-glucosidase inhibitory activity of extracts from six varieties of Cucurbita moschata Duch.

目的:对6种不同栽培品种南瓜(金钩、甜面、日本、辽宁新民金钩、超甜蜜本、蜜本)提取物α-葡萄糖苷酶抑制活性进行研究。方法:通过建立体外α-葡萄糖苷酶抑制模型,对南瓜提取物进行活性筛选,并对提取物浓度与抑制活性关系进行研究。结果:6种栽培品种南瓜不同溶剂提取物均有一定的α-葡萄糖苷酶抑制活性,其中,金钩南瓜石油醚提取物的抑制活性最好(IC50=143.91μg/mL),活性远大于阳性对照阿卡波糖(IC50=1103.01μg/mL)。不同溶剂提取物显示石油醚提取物抑制活性均高于乙酸乙酯提取物和甲醇提取物。结论:6种栽培品种南瓜提取物均有一定的α-葡萄糖苷酶抑制活性,但不同品种其抑制活性具有一定的差别。

Objective： To study the α-glucosidase inhibitory activity of extracts from six varieties of Cucurbita moschata Duch. （Jingou, Tianmian, Riben, Chaotianmiben, Miben and Liaoningxinminjingou ） . Methods. By established α-glucosidase inhibitory model in vitro,the activity of extracts from C.moschata was screened.The relationship of inhibitory ratio and the extract concentration were also studied. Results： Different extracts from six varieties of C.moschata all had inhibitory activity of a-glucosidase, among which petroleum ether extract of C.moschata（Jingou） had the highest inhibitor activity of α-glucosidase （IC50 = 143.91μg/mL）, which was lower than that of Acarbose（ IC50 = 1103.0μg/mL）.Among different extracts inhibitor activity of petroleum ether extract was higher than that of ethyl acetate extract and methanol extract.Conclusion：The extracts from six varieties of C. moschata all had inhibitory activity of α-glucosidase, but different varieties of the inhibition rate had certain difference.