蛋氨酸羟基类似物对肉鸡肠道氧化还原状态的影响

Effect of 2-hydroxy-4-(methylthio) butanoic acid on intestinal redox state of broiler chickens

目的:研究蛋氨酸(DLM)、蛋氨酸羟基类似物(HMTBA)及其钙盐(HMTBA-Ca)对肉鸡肠道氧化还原状态的影响。方法:1日龄罗斯肉鸡(44g)随机分为对照组(基础日粮)和实验组(在基础日粮中分别添加0.1%和0.2%的DLM、HMTBA及HMTBA-Ca),共7个处理组。结果:与对照组相比,0.1%HMTBA组和0.1%HMTBA-Ca组可显著降低全血ROS水平,提高肠道GSH/GSSG和T-AOC,降低血浆及组织MDA含量。0.2%HMTBA组和0.2%HMTBA-Ca组可显著提高血浆和组织CAT活性。基础日粮中添加HMTBA和HMTBA-Ca均可使肠绒毛高度升高、隐窝深度下降、V/C升高。此外,0.1%HMTBA组和0.1%HMTBA-Ca组ROS水平及MDA含量均低于0.1%DLM组,肠绒毛高度和V/C则高于0.1%DLM组。结论:与DLM相比,HMTBA及HMTBA-Ca能更好地提高肠道抗氧化能力,降低ROS水平,维持肠道氧化还原稳态,促进肠道健康发育。

Objective ：To research the effects of methionine sources methionine （ DLM ）, 2- hydroxy-4- （methylthio） butanoic acid （HMTBA） and 2-hydroxy-4- （methylthio） butyrate calcium （HMTBA-Ca） on intestinal redox state of broiler chickens.Method ： One hundred and five （ 105 ） broiler chickens （44g） were randomly divided into 7 groups （15 birds per group） ,and each group was assigned to 1 of 3 treatments.Birds in each group were kept in three coops（5 broilers per coop）.AII broilers were offered the same basal diet with added DLM,HMTBA and HMTBA- Ca at levels of 0 （control group）, 0.1% and 0.2%. Results： Compared with control group, basal diet with 0.1% HMTBA group and 0.1% HMTBA-Ca group could decrease the level of free radicals in whole blood significantly, enhance the activity of GSH/GSSG, T-AOC of the serum and tissues significantly, decrease the level of MDA significantly.Compared with control group,basal diet with 0.2% HMTBA group and 0.2% HMTBA-Ca group could enhance the activity of CAT of the serum and tissues significantly.Basal diet with added HMTBA, HMTBA-Ca could enhance the intestinal villus height（V）and the ratio of the villus height to recess depth of broilers（V/C） ,decrease the intestinal recess depth （C）of broilers significantly.Compared with 0.1% DLM group, ROS, MDA levels were lower and V,V/C were higher in basal diet with 0.1% HMTBA group and 0.1% HMTBA-Ca group.Conclusion： Compared with DLM, HMTBA and HMTBA-Ca could better increase the intestinal antioxidative capacity,decrease the level of free radicals,steady intestinal redox state, promote the intestinal development healthily.