岷山红三叶草异黄酮合酶基因的克隆及序列分析

Cloning and sequence analysis of isoflavones synthase gene from Trifolium Pratense Minshan

从岷山红三叶克隆异黄酮合酶基因,并对序列进行比对和进化分析。建立岷山红三叶愈伤组织培养体系,采用RT-PCR扩增从愈伤组织总RNA中分离了异黄酮合酶基因,并将其克隆到pGEM-T Easy载体,测序,得到全长1575bp的片段。序列分析表明,异黄酮合酶基因含1575个核苷酸,和大豆等其他植物中异黄酮合酶具有很高的同源性。

For making good use of the specific biological function of isoflavone.A cDNA fragment from full length IFS gene was amplified by polymerase chain reaction from total RNA of callus Trifolium Pratense Minshan, and it was sequenced after cloned into pGEM-T Easy vectors.A cDNA fragment of 1575bp from full length IFS gene was got. The sequencing results indicated that the cloned fragment of IFS contained 1575 nucleotides, and shared a sequence homology of 99% with that from Genbank accession.