香附挥发油对吡罗昔康体外促透皮作用研究

Enhancing Percutaneous Permeability of Piroxicam through Rat Skin by Essential Oil from Cyperus rotundus L.

目的考察香附挥发油对吡罗昔康体外经SD大鼠皮渗透的影响,为开发中药透皮促进剂和吡罗昔康经皮渗透剂提供依据。方法用改良的Franz扩散池进行体外透皮实验,HPLC法测定吡罗昔康含量。结果浓度大于1%的香附挥发油,对吡罗昔康具有良好的促渗透作用。吡罗昔康中加入5%香附挥发油、5%氮酮后的累积渗透量及增渗倍数分别为(188.9±19.62)μg/cm2、(268.4±28.81)μg/cm2和6.156、6.611。结论香附挥发油对吡罗昔康的体外渗透作用具有明显的促进作用。

Objective To study the effects of essential oil from Cyperus rotundus L.on percutaneous permeability of piroxicam through rat shin in vitro,and provide the basis for screening penetration enhancers form traditional Chinese medicine and developing the transdermal formulation of piroxicam.MethodTransdermal test was performed by the modified Franz diffusion cell in vitro and the cumulative permeation content of piroxicam was determined by HPLC.Results A good percutaneous permeability was obtained when the essential oil from Cyperus rotundus L.was more than 1%.The Cumulative permeation amount and the enhanced permeability factors of piroxicam with 5% Cyperus rotundus volatile oil and 5% azone were（188.9±19.62） μg/cm2、（268.4±28.81） μg/cm2 and 6.156、6.611.Conclusion The essential oil from Cyperus rotundus L.shows strong penetration enhancing effect for piroxicam.