原发性肝癌尿激酶和尿激酶受体表达的研究

Studies on the expression of urokinase and its receptor in hepatocellular carcinoma

目的 研究肝癌尿激酶和尿激酶受体的表达 ,探讨肝癌侵袭和转移分子机制。方法 应用酶联免疫吸附试验 (ELISA)和放射配基结合试验分析 12例肝癌及癌旁组织中尿激酶和尿激酶受体的表达。结果 肝癌组织中尿激酶水平为 (777± 5 86 ) pmol/g蛋白 ,细胞表面尿激酶受体数量为(6 49± 2 2 3) pmol/ g蛋白 ,均显著高于癌旁组织 [(84± 5 7)pmol/ g蛋白和 (72± 30 ) pmol/g蛋白 ,P <0 .0 1];肝癌细胞尿激酶与尿激酶受体的高亲和力 (0 .33± 0 .18)比癌旁组织 (6 .6 3± 2 .17)更高 (P <0 .0 1)。结论 肝癌组织中尿激酶与尿激酶受体过量表达 ,这可能在肝癌的侵袭和转移过程中起重要作用。

Objective\ To study the expression of urokinase and its receptor to investigate the molecular mechanism of invasion and metastasis of hepatocellular carcinoma (HCC). Methods\ By using ELISA and rediolegent binding assay, urokinase (uPA) and its receptor (uPAR) were detected in 12 specimens from tumor of HCC and adjacent liver tissues. Results\ The level of uPA and the amount of uPAR in HCC were increased significantly as compared with those in the adjacent liver tissues [(777±586) pmol/g protein, (649±223) pmol/g protein vs (84±57) pmol/g protein, (72±30) pmol/g protein, P <0.01]. The uPAR on the surface of HCC cells was expressed with higher affinity than that in the adjacent liver tissues (0.33±0.18) vs (6.63±2.17), ( P <0.01). Conclusion\ uPA and uPAR were overexpressed in HCC, which might play an important role in the process of invasion and metastasis of HCC.\;