联合使用库司托啶与异搏定对大鼠肝脏低温保存的作用

The protective effects of cold preservation of liver with custodiol solution and verapamil

目的 研究库司托啶 (Custodiol)与异搏定 (Verapamil)联合应用对低温保存大鼠肝脏的保护作用。方法 将 40只Wistar大白鼠制作肝离体灌注模型 ,库司托啶和异搏定作肝脏灌注并置于 4℃保存 6、2 4和 48h后观察肝窦内皮细胞死亡率、肝功能以及肝细胞形态的变化。结果 库司托啶和异搏定灌注组明显优于对照组 ,保存 2 4h ,库司托啶 +异搏定 (CVA)组、库司托啶组的肝窦内皮细胞死亡率分别为 30 .2 9%和 36 .36 % ,低于异搏定组 (5 7.5 3 % )和林格氏液组 (6 1.5 5 % ) ,其差异有显著性 (P <0 .0 5 )。保存 48h ,CVA组 (4 9.46 % )、库司托啶组 (5 7.45 % )与异搏定组 (78.79% )、林格氏液组 (89.2 1% )之间的差异增大 ,其差异有非常显著性 (P <0 .0 0 1)。两者联合应用优于单独应用。

Objective\ To study the protective effect of cold preserved rat liver with custodiol solution and verapamil. Methods\ The isolated perfusion liver model of 40 Wistar rats was used. The liver was perfused with custodiol solution and verapamil. After 6 h, 24 h and 48 h of preservation at 4℃ the mortality of sinusoidal endothelial cell and the LDH, ATL activities were observed. Results\ The effect of groups of custodiol solution and verapamil was better than in the group of control. After 24 h preservation, the mortality of hepatic sinusoidal endothelial cell in group CVA and group custodiol was 30.29% and 36.36% , which were lower than in group verapamil (57.53%) and group Ringer's (61.55%). There was a singnificant difference ( P <0.05). After 48 h preservation,the differences bettween group CVA ( 49.46% ), group custodiol (57.45%) and group verapamil(78.79%) or group Ringer's (89.21%) were the most significant ( P <0.001). Conclusion\ The combination of verapamil and custodiol solution had an obvious protective effect on donor rat liver. \;