间氯苯哌嗪诱导的小鼠明暗箱焦虑模型──一种新的经济简便抗焦虑药筛选模型

m -CHLOROPHENYLPIPERAZINE-INDUCED ANXIETY MODEL IN LIGHT-DARK BOX IN DDY MICEA NEW ECONOMIC AND SIMPLE METHOD FOR SCREENING ANXIOLYTICS

目的 建立经济简便的抗焦虑药模型。方法 用间氯苯哌嗪 (mCPP)诱导产生焦虑 ,观察ddy小鼠和ICR小鼠在明暗箱的行为表现。结果 mCPP在sc 1～ 4mg·kg-1的剂量下即可显著降低ddy小鼠在明箱的活动次数 ,而对暗箱的活动次数影响不显著 ;mCPP在sc 2～ 10mg·kg-1的剂量下对ICR小鼠在明箱的的活动次数影响不明显 ;用地西泮对该模型进行验证 ,发现只用较小的样本量即可得出显著结果。结论 ddy小鼠可取代Wistar大鼠进行mCPP诱导焦虑的明暗箱模型 ,且简便易得 ,经济有效。

AIM To develop a new economic and simple anxiety model that mimics clinical conditions for screening and studying anxiolytic agents. METHODS The behavior activity of ddy and ICR mice in light-dark box was observed after sc m-chlorophenylpiperazine( m CPP) to induce anxiety. RESULTS m CPP was shown to significantly decrease the activity of ddy mice in light box after sc 1～4 mg·kg -1 , but the activity in dark box was not significantly changed. No significant difference was found in the light box between the 1 mg·kg -1 and the 4 mg·kg -1 group. m CPP showed little effect on the activity of ICR mice in the light box, but it was shown to significantly decrease the activity in the dark box between 2～10 mg·kg -1 . It is easy to show the anxiolytic effect of diazepam only in a small sample by using ddy mice in this model. CONCLUSION It is easy to get effective result by using only a small sample in the light-dark box after m CPP-induced anxiety in ddy mice. It appears that this model is an economic and simple anxiety model that mimics clinical conditions for screening and studying anxiolytic agents.