AFLP法构建人参、西洋参基因组DNA指纹图谱

CONSTRUCTION OF GENOMIC DNA FINGERPRINTING IN PANAX GINSENG AND P.QUINQUEFOLIUM BY AFLP

目的 采用扩增片段长度多态性DNA(AFLP)分子遗传标志技术 ,分析人参、西洋参基因组DNA多态性。方法 人参、西洋参干燥根基因组DNA ,经EcoRI/MseI酶切并与其相应的人工接头连接后 ,使用选择性引物进行PCR扩增。结果 经变性聚丙烯酰胺凝胶电泳检测 ,成功构建出多态性丰富和重复性好的人参、西洋参DNA指纹图谱。结论 AFLP法有望成为一种独立的切实可行的手段 ,将在人参、西洋参等药用植物的鉴定、生物进化、系统发育研究及指导道地性药材的科学栽培等方面发挥重要作用。

AIM To analyse the genetic polymorphisms of Panax ginseng and P.quinquefolium by amplified fragment length polymorphism DNA (AFLP) molecular genetic marker technique. METHODS Genomic DNAs extracted from dried roots of Panax ginseng and P.quinquefolium were digested by EcoRI in combination with MseI and ligated with EcoRI and MseI adapters, respectively. Meanwhile, the polymerase chain reactions were performed with E-AA and M-CAG selective primer pairs. RESULTS The distinct, reproducible genomic DNA fingerprints were constructed using denatured polyacrylamide gel electrophoresis assay. CONCLUSION AFLP, a powerful tool, was shown to play important roles in authentication, biological evolution, phylogenetic development of medicinal plant materials, including Panax ginseng and P.quinquefolium , and in guidance of genuine medicinal materials cultivation.