凤尾鸡冠的离体快繁及瓶苗开花探究

Rapid Propagation in Vitro and Flowering of Test-tube Plantlets of Celosia Crtstata L.cv. Plumosa

应用植物离体快繁培养技术研究凤尾鸡冠瓶苗开花技术,结果表明:凤尾鸡冠的种子用0.1%的HgCl2灭菌8 min效果最好,成活率达到90%;最佳的增殖培养基配方为MS+NAA 0.1 mg·L-1+6-BA 1.0 mg·L-1+蔗糖20 g·L-1+琼脂10 g·L-1;经过壮苗后的植株在MS+PP3330.5 mg·L-1+NAA 0.01 mg·L-1+蔗糖20 g·L-1+琼脂10g·L-1培养基上培养,开花率最高,为81%;适当减少MS培养基中氮含量,能提高凤尾鸡冠瓶苗开花率;光照强度为3 000 Lx时,瓶苗开花率最高。

The flowering of test-tube plantlets of Celosia crtstata L. cv. Plumosa was studied by using vitro tissue culture method of rapid propagation. The results showed that seeds of Celosia crtstata L.cv. Plumosa disinfected in 0.1% HgC12 for 8 minutes achieved the highest survival percentage （90%）. The best medium for plantlet proliferation was MS＋NAA 0.1mg＇L t＋6-BA 1.0mg.L-t＋sucrose 20g·L^-1＋agar 10g·L^-1. The MS medium supplemented with PP333 0.5mg·L^-1＋NAA 0.01 mg·L^-1＋sucrose 20g·L^-1＋agar 10g·L^-1 was found to be the best for strong seedling flowering with flowering rate up to 81%. It was suggested that decreasing the content of nitrogen element in MS medium properly would enhance the flowering rate. It reached the highest flowering rate when the light intensity became 3000Lx.