津南芹菜EST-SSR指纹图谱的构建及遗传差异分析

Construction of SSR-based Molecular Fingerprinting and Analysis of Genetic Diversity for Celery Varieties from Tianjin

以天津市津南11个芹菜育种材料为研究对象,利用EST-SSR分子标记技术构建津南芹菜分子指纹图谱,并用于遗传多样性分析。从GenBank数据库查询芹菜EST序列1122条,使用BatchPrimer3在线软件扫描分析获得194个候选EST-SSR位点,并设计141对SSR-PCR引物。选择其中47对引物进行多态性分析,确认8对引物具有多态性。构建基于Excel格式的津南芹菜EST-SSR指纹图谱,包括8对引物在供试材料中扩增到的44条多态性条带信息。遗传多样性分析显示,供试材料间遗传相似系数介于0.409～0.864之间,平均为0.664。本研究建立的津南芹菜EST-SSR指纹图谱稳定有效,可用于芹菜种质资源鉴定和品种真实性检测。

SSR markers were used to construct the fingerprinting and genetic diversity of 11 celery varieties from Jinnan, Tianjin city.A total of 1 122 celery EST were hunted from the Genebank database.114 microsatellites sequences containing 194 EST-SSR were detected from these EST and 147 primer pairs were designed by BatchPrimer3 online software.A subset （47 pairs） was synthesized to test the amplification and polymorphism in 11 celery cultivars.The results showed that 8 primer pairs presented genetic diversity. The Excel-based digital fingerprinting was established.UPGMA cluster analysis was used to estimate the genetic distances and to construct a dendrogram by SLT-NTSYS.The results showed that the coefficient of genetic similarity range was 0.409~0.864,with an average of 0.664.The celery EST-SSR fingerprinting was reliable and convenient,and could be used in germplasm identification.