皖南地区汉族ICP患者ERα基因多态性的研究

ERα gene polymorphism of intrahepatic cholestasis of pregnancy in Chinese Han population from south Anhui province

目的:探讨雌激素受体α(ERα)基因多态性在妊娠期肝内胆汁淤积症(ICP)发病中的作用。方法:应用PCR-限制性片段长度多态性(PCR-RFLP)和DNA序列测定方法,研究43例ICP患者(ICP组)和93例正常孕妇(对照组)中ERα基因PvuⅡ多态性分布情况。结果:ERα基因PvuⅡ基因型(PP、Pp和pp)分布在ICP组和对照组之间差异无显著性(χ2=4.762,P=0.092)。等位基因P、p频率在这两组之间差异也无显著性(χ2=2.385,P=0.122)。结论:ERα基因多态性与ICP发生可能无明显相关性。

Objective：To examine the effect of estrogen receptor alpha（ERα）gene polymorphisms in the intrahepatic cholestasis of pregnancy（ICP）.Methods： The distribution of ERα gene PvuⅡ polymorphisms in 43 pregnant women with ICP（ICP group）and 93 normal pregnant women（control group）was determined by polymerase chain reaction-restriction fragment length polymorphis（PCR-RFLP）and DNA sequencing.Results： No significant difference was seen regarding the distribution of PvuⅡ genotypes（PP,Pp and pp） of the ERα gene between ICP group and control group（χ2=4.762,P=0.092） as well as the frequency of P,p alleles between the two groups（χ2=2.385,P=0.122）.Conclusion： There may be no significant correlation between ERα gene polymorphisms and the ICP in Chinese Han population from south Anhui province.