玉米热激蛋白基因ZmHsp90-1的克隆及表达分析

Cloning and Expression Analysis of Heat Shock Protein Gene ZmHsp90-1 in Maize

Hsp90是普遍存在于原核和真核细胞中的一种高度保守的分子伴侣。本研究从玉米中克隆了一个Hsp90同源基因,命名为ZmHsp90-1基因,并对其进行了初步的序列分析。该基因cDNA序列全长2371bp,开放阅读框2094bp,编码697个氨基酸,蛋白质分子量约79.98kD。蛋白结构预测及同源比对分析表明,ZmHsp90-1基因编码蛋白含ATPase位点和Hsp90保守结构域,并与拟南芥、水稻等多种物种的热激蛋白高度同源;进化树分析表明ZmHsp90-1与拟南芥AtHsp90.1基因关系较近,蛋白序列相似性达88.3%。目的蛋白亚细胞定位显示,ZmHsp90-1蛋白在细胞质中表达。实时荧光定量PCR分析表明,ZmHsp90-1对非生物胁迫高温、高盐、ABA、低温、干旱均具有明显的应答反应。推测ZmHsp90-1是玉米的一个胁迫相关基因。

The heat shock protein 90 （Hsp90） is a widespread family of molecular chaperones found in prokaryotes and all eukaryotes, with high conservation among plant species. In this study, a Hsp90 gene was isolated from maize, named ZmHsp90-1. The full cDNA sequence of ZmHsp90-1 is 2 371 bp, containing a 2 094 bp open reading frame （ORF） and encoding 697 amino acids with a predicted molecular mass of 79.98 kD. The ZmHsp90-1 protein contains a predicted ATPase site and a Hsp90 conservative structure domain, which is highly conserved in plants and especially similar to AtHsp90.1. Instantaneous expression analysis showed that ZmHsp90-1 proteins was localized in cytoplasm. The expression of ZmHsp90-1 in maize analyzed by quantitative real-time PCR was induced by hot, high-salt, ABA, cold and drought treatments. These results suggested that ZmHsp90-1 might be a stress related gene of maize.