吡格列酮联合放射治疗对小鼠结肠癌的抑制作用

Effects of pioglitazone combined with radiotherapy on colon carcinoma xenograft in mice

目的探讨过氧化物酶体增殖物激活受体γ（PPARγ）配体吡格列酮联合放射治疗对小鼠结肠癌的抑制作用。方法将小鼠结肠癌细胞CT26接种于BALB／C小鼠右侧肋腰部，将小鼠按完全随机数字表法随机分为空白对照组、肿瘤对照组、溶剂二甲基亚砜对照组、吡格列酮组、放疗组和放疗＋吡格列酮组，每组30只，分别观察各组肿瘤生长情况，计算并比较抑瘤率，病理观察肿瘤在光学显微镜下的变化。结果与肿瘤对照组小鼠肿瘤体积比较，放疗后2周吡格列酮组、放疗组、放疗＋吡格列酮组对小鼠结肠癌的生长均有抑制作用（P=0．008、0．001、0．001），放疗组或放疗＋吡格列酮组与吡格列酮组比较，差异均有统计学意义（P=0．026、0．018），放疗组与放疗＋吡格列酮组比较，差异无统计学意义（P=0．335）；吡格列酮组、放疗组、放疗＋吡格列酮组的抑瘤率分别为46．30％、68．60％和70．01％；肿瘤病理学变化明显。结论PPARγ配体吡格列酮、放疗以及两者联合均能够抑制小鼠结肠癌的生长，PPARγ是肿瘤治疗较好的新靶点。

Objective To investigate the effects of peroxisome proliferator activated receptor T （PPARγ） agonists pioglitazone combined with radiotherapy on colon carcinoma xenograft in mice. Methods The colon cancer cells CT26 of mouse were inoculated in the right side of the rib loins of BALB/c mouse. Established BALB/c mice tumor models. The mice were randomly divided into six groups： untreated group, tumor contral group, DMSO group, pioglitazone treated group, radiotherapy group, radiotherapy combined with pioglitazone treated group. To observe the tumor growth of each group, to compute and compare to the tumor inhibition rates, the pathological varieties of the tumor were observed. Results The tumor volumes were compared with each group after two weeks of radiotherapy.Compared with tumor contral group, pioglitazone treated group, radiotherapy group and radiotherapy combined with pioglitazone treated group could decrease the volume of tumor （P =0.008, P = 0.001, P = 0.001）. Compared with pioglitazone treated group, the effect of radiotherapy group or radiotherapy combined with pioglitazone treated group was more evident （P = 0.026, P = 0.018）. Contrasted with radiotherapy group, radiotherapy combined with pioglitazone treated group had no significant difference （P = 0.335）. The tumor inhibition rates of pioglitazone treated group, radiotherapy group and radiotherapy combined with pioglitazone treated group were 46.30 %, 68.60 % and 70.01%. The pathological varieties of tumor were obvious. Conclusion Pioglitazone, radiotherapy and radiotherapy combined with pioglitazone could inhibit the growth of tumor. PPARγ, is a new target for tumor treatment.