杏香兔耳风颗粒中绿原酸含量测定研究

Determination of Chlorogenic Acid in Xingxiang Tu-erfeng Granules

目的:建立杏香兔耳风颗粒中绿原酸的含量测定方法。方法:色谱柱为Diamonsil C18(2)柱(4.6 mm×250 mm,5μm),以甲醇-2%冰醋酸(27:73)为流动相,检测波长327nm,结果:绿原酸的线性范围内为7.72μg mL-1-193.00μg mL-1,相关系数r均为0.9999;回收率为101.23%(RSD=2.48%)。结论:本方法稳定、重现性好,可作为本制剂的含量测定方法。

Objective：To establish a method for determination chlorogenic acid in Xingxiang Tu-erfeng granules.Methods： Chromatogra phy was performed on a Diamonsil C18（2） column（4.6 mm ×250 mm,5μm）.The mobile phase was methanol-2%HAC（27：73）,the detection wavelength was UV 327 nm respectively.Results： The linear ranges of the determination of chlorogenic acid is 7.72 μg/ mL～193 μg/mL（r=0.9999）;the average recovery was 101.23%,RSD was 2.48%.Conclusion： The method is Stable and reliable,suitable for the quality control of the medicine.