补肾口服液中淫羊藿苷含量的HPLC测定方法研究

A Methodological Study of the Quantitative Determination of Icariin in Busen Oral Liquid by HPLC

目的：建立补肾口服液中淫羊藿苷的高效液相色谱定量方法。方法：采用ZORBAX SB-C18（4.6mm×250mm,5μm）色谱柱,流动相为甲醇-水（63：37）,流速1.0 mL/min,检测波长为270nm,柱温25℃。结果：淫羊藿苷浓度为0.0224~0.448mg范围内线性关系良好（r=0.9995）,平均加样回收率为100.08%,RSD为1.25%（n=6）。结论：该方法简便、准确、灵敏度高、专属性强、重现性好,可作为补肾口服液的质量控制方法。

Objective： To establish an HPLC method for detemination of Icariin in busen oral liquid.Method： The separation was per formed on a ZORBAX SB-C18 column（4.6mm× 250mm,5μm）.The mobile phase was CH3OH-H2O（63：37）.gradient elution at flow rate of 1mL/min,the eluent monitored by a DAD detector at 270 nm,The column temperature was 25℃.Result： The linear range of Icariin was 0.0224~0.448mg,r=0.9995。The average recovery was 100.08%,RSD=1.25%。Conclusion： The method is simple,ac curate,sensitive,specific and reproducible.it can be used for quality evaluation and quality control of busen oral liquid.