肽聚糖通过TLR2诱导人绒毛外滋养细胞的凋亡及意义

Peptidoglycan induces TEV-1 cells apoptosis through Toll like receptor 2

目的初步研究肽聚糖通过Toll样受体2(TLR2)诱导人早孕绒毛外滋养细胞株TEV-1细胞的凋亡及其意义。方法免疫细胞化学检测TLR2蛋白在TEV-1细胞的定位表达,不同浓度肽聚糖刺激TEV-1细胞后AnnexinV/PI流式细胞术检测细胞的凋亡率。结果①TEV-1细胞表达TLR2,且定位于细胞膜和细胞浆。②30μg/ml肽聚糖刺激TEV-1细胞24小时后与对照组比较,细胞凋亡率无明显增加(8.17±0.1%,P>0.05),而刺激时间延长至48h可诱导出明显增加的细胞凋亡(16.03±1.51%,P<0.01);大剂量组80μg/ml肽聚糖刺激TEV-1细胞24h、48h均可诱导明显增加的细胞凋亡(14.13±1.06%和51.67±1.56%,P<0.01)。结论人绒毛外滋养细胞株TEV-1表达TLR2,肽聚糖可通过TLR2诱导TEV-1细胞凋亡,且存在时间和剂量依赖性,提示宫内感染时G+菌可能通过TLR2介导胎盘滋养细胞凋亡来影响妊娠的结局。

Objective： To investigate the significance of the apoptosis induced by peptidoglycan（PGN）.in the human extravillous trophoblast cell line（TEV-1） Methods：The expression of Toll-like receptor-2（TLR2） in TEV-1 cells were detected by immunocytochemistry SP staining.TEV-1 cells were incubated with different dose peptidoglycan,the flow cytometry.was used to measure apoptosis.Results：①TLR2 was expressed in TEV-1 cells and localized to both the cytoplasm and plasma membrane.②Compared with the untreated control,incubated with 30μg/ml PGN for 24h didn＇t increase apoptosis in TEV-1 cells（8.17±0.1%,P〈0.05）,but when it last to 48h,there was a significant increase in apoptosis cells.（16.03±1.51%,P〈0.01）.Incubated with 80μg/ml PGN for 24h and 48h could both increase apoptosis in TEV-1 cells（14.13±1.06% and 51.67±1.56%,P〈0.01）.Conclusion：TLR2 was expressed in TEV-1 cells.PGN does act to induce apoptosis in TEV-1 cells through TLR2 and that this effect is both dose-and time-dependent.That suggests intrauterine infections may cause the excessive trophoblast cells apoptosis through TLR2.and affect the result of pregnancy.