氯嘧磺隆高效降解菌2N3的生长特性研究

Studies on Growth Characteristics of Highly Efficient Degrading Strain 2N3 of Chlorimuron-Ethyl

本文对氯嘧磺隆高效降解菌2N3的生长特性进行了研究。结果表明:供试碳氮源中,葡萄糖为碳源时,5×10-3g/L氯嘧磺隆96 h时降解完全;蛋白胨为氮源时,氯嘧磺隆96h时的降解率仅为5%,因此葡萄糖为最优碳源;同时在2N3最优生长环境正交试验中,当葡萄糖添加浓度5%,接菌量1%,氯化钠0.2%,通气量100 mL条件下,培养18 h时2N3生长量最大。

Growth characteristics of the Klebsiellasp. 2N3 strain in chlorimuron-ethyl were examined in the paper. The results showed that when glucose was carbon source, 5 x 10^-3 g/L chlorimuron-ethyl was degraded completely after 96 hours, while only 5% of chlorimuron-ethyl was degraded when peptone was the nitrogen source. So glucose was the best carbon source. Optimal environment for the growth orthogonal test of 2N3 showed that concentration of glucose was 5%, the inoculate of 2N3 1% ,sodium chloride 0.2% ,optimal ventilation 100 mL,after inoculating 18 h, OD6oo was up to 0.8 and growth of 2N3 was the most.