摘要
目的:观察慢盆消炎方对慢性子宫内膜炎(CE)模型大鼠IL-2、MMP-2及TIMP-2的影响。方法:采用混合菌株联合支原体接种法制作慢性子宫内膜炎大鼠模型,给予慢盆消炎方灌胃治疗,观察大鼠子宫及内膜组织病理改变,检测血清IL-2水平及子宫组织中基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶抑制剂-2(TIMP-2)的表达差异。结果:慢盆消炎方能改善慢性子宫内膜炎模型大鼠子宫的组织损害,提高血清IL-2含量,降低子宫内膜组织MMP-2表达及调节MMP-2/TIMP-2比值。结论:慢盆消炎方对CE模型大鼠具有显著治疗作用,减少细胞外基质(ECM)和血管基底膜降解是其作用机制。
Objective： To investigate the therapeutic effect and mechanisms of Manpenxiaoyan Decoction （MPXYD） on rat with chronic endometritis. Methods： Rat models with chronic endometritis were established by inoculating admixed bacteria into endometrium and mycoplasma into vagina. Seventy SD model rats were randomly divided in- to 7 groups： blank control group, model group, Fulechongji group, doxycycline group, and low-,medium-,and high-dose MPXYD groups,10 in each. Each group was intragastrically given the corresponding agents. Pathologi- cal changes of the endometrium were observed. The serum level of IL-2 and matrix metalloproteinase-2 （MMP-2） and tissue inhihitors of meralloproteinase-2（TIMP-2） in endometrial tissue were determined. Results： MPXYD reduced inflammatory cell infiltration in endometrium, elevated IL-2,and reduced the expression of MMP-2 and the ratio of MMP-2 to TIMP-2. Conclusion： MPXYD may cure chronic endometritis by reducing in- flammation and degrading basement membrane of blood vessels.
出处
《浙江中西医结合杂志》
2012年第10期762-764,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
