山东阿胶膏的质量标准研究

Study on Quality Standard of Shandongejiao Concentrated Decoction

目的:完善山东阿胶膏的质量标准。方法:采用薄层色谱法对山东阿胶膏中阿胶和甘草进行定性鉴别;用高效液相色谱-蒸发光散射检测器法(HPLC-ELSD)测定黄芪中黄芪甲苷的含量。结果:应用所建立的方法对样品进行定性与定量分析,结果在TLC色谱中均可检出与对照品或对照药材相同的斑点,且阴性无干扰;黄芪甲苷进样量在1.032～6.192μg范围之间其对数值与峰面积对数值呈良好的线性关系,平均加样回收率为100.51%,RSD为1.43%。结论:该方法操作简便,重现性好,结果准确可靠,可用于山东阿胶膏的质量控制。

Objective： To improve the quality standard of shandongejiao Concentrated Decoction. Method： TLC was used to identify Asini Corii Colla and Glycyrrhizae Radix et Rhizoma, and HPLC-ELSD to determinate Astragaloside IV of Astragali Radix. Result： Asini Corii Colla and Glycyrrhizae Radix et Rhizoma could be identified, and the blank test showed that other medicine herbs had no interference. There was a good linear relationship in the range of 1. 032 - 6. 192μg for astragaloside IV. The average recovery was 100. 51% , RSD = 1.43% （n =6）. Conclusion：The method was simple, accurate and reproducible, and can be used for the quality control of Shandongejiao Concentrated Decoction.