摘要
目的:探讨髓样细胞触发受体-1(TREM-1)基因和高迁移率族蛋白B-1(HMGB-l)在重症急性胰腺炎大鼠肺组织中的表达及作用。方法:选择健康成年雄性SD大鼠72只,随机分为对照组(A组,36只)和模型组(B组,36只),采用5%牛磺胆酸钠逆行胰胆管注射法建立重症急性胰腺炎大鼠模型,在建模后6h、24h分别观察光镜下肺组织形态,以肺湿重/干重检测肺含水量,采用酶联免疫吸附试验(ELISA)测定肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平、HMGB-1含量,采用半定量逆转录-聚合酶链反应技术(RT-PCR)测定肺组织TREM-1mRNA水平。结果:与A组比较,B组的肺损伤程度明显加重,并呈持续加重趋势;B组的肺水含量较A组增多;B组肺组织TNF-α、IL-1β、HMGB-1水平、TREM-1mRNA的表达明显增高,且TREM-1mRNA、HMGB-1持续升高,差异具有统计学意义(P<0.05)。结论:TREM-1mRNA及HMGB-1与重症急性胰腺炎并发肺损伤的发生与发展明显相关。
Objective To investigate the expression and the possible effects of triggering receptor expressed on myeloidcells-l(TREM-1) and high mobility group box-1 protein (HMGB-1) in rat lung tissue with severe acute pancreatitis. Methods 72 male SD rats were randomly divided into two groups: control group(groupA) and severe acute pancreatitis model group(group B). Severe acute pancreatitis animal model was induced by injecting of 5% sodium taurocholate into biliopancreatic ducts retrogradely with fine needle. Rats in each group were sacrificed at 6 h, 12 h, 24 h, after operations respectively. The pathological changes of lung were observed by lightmicroscopy, the ratio of wet weight to dry weight of lung was detected. The concentrations of TNF-α, IL-1 β and HMGB-1 were measured by the method of ELISA. The expression of TREM-1 mRNA of lung tissue was detected by RT-PCR. Results Compared with group A, the extent of lung damage was severer and it lasted a growing trend in group B, the water content of lung tissue in group B was increased. Simultaneously, the levels of TNF- α, IL-1 β, HMGB-1 and the expression of TREM-1 mRNA of lung tissue in group B were significantly higher, and the levels of TREM-1 mRNA and HMGB-1 were heightened duratively(P〈0.05). Conclusion The levels of TREM-1 mRNA and HMGB-1 are positively correlated with the severity of severe acute pancreatitis associated lung injury.
出处
《深圳中西医结合杂志》
2012年第5期273-276,F0003,共5页
Shenzhen Journal of Integrated Traditional Chinese and Western Medicine
基金
深圳市科技计划资助项目(201003032)