TREM-1基因和高迁移率族蛋白B-1在大鼠重症急性胰腺炎肺组织中的表达及作用

Expression and the Possible Effects of TREM-1 and HMGB-l in Rat Lung Tissue with Severe Acute Pancreatitis

目的:探讨髓样细胞触发受体-1(TREM-1)基因和高迁移率族蛋白B-1(HMGB-l)在重症急性胰腺炎大鼠肺组织中的表达及作用。方法:选择健康成年雄性SD大鼠72只,随机分为对照组(A组,36只)和模型组(B组,36只),采用5%牛磺胆酸钠逆行胰胆管注射法建立重症急性胰腺炎大鼠模型,在建模后6h、24h分别观察光镜下肺组织形态,以肺湿重/干重检测肺含水量,采用酶联免疫吸附试验(ELISA)测定肺组织肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)水平、HMGB-1含量,采用半定量逆转录-聚合酶链反应技术(RT-PCR)测定肺组织TREM-1mRNA水平。结果:与A组比较,B组的肺损伤程度明显加重,并呈持续加重趋势;B组的肺水含量较A组增多;B组肺组织TNF-α、IL-1β、HMGB-1水平、TREM-1mRNA的表达明显增高,且TREM-1mRNA、HMGB-1持续升高,差异具有统计学意义(P<0.05)。结论:TREM-1mRNA及HMGB-1与重症急性胰腺炎并发肺损伤的发生与发展明显相关。

Objective To investigate the expression and the possible effects of triggering receptor expressed on myeloidcells-l（TREM-1） and high mobility group box-1 protein （HMGB-1） in rat lung tissue with severe acute pancreatitis. Methods 72 male SD rats were randomly divided into two groups： control group（groupA） and severe acute pancreatitis model group（group B）. Severe acute pancreatitis animal model was induced by injecting of 5% sodium taurocholate into biliopancreatic ducts retrogradely with fine needle. Rats in each group were sacrificed at 6 h, 12 h, 24 h, after operations respectively. The pathological changes of lung were observed by lightmicroscopy, the ratio of wet weight to dry weight of lung was detected. The concentrations of TNF-α, IL-1 β and HMGB-1 were measured by the method of ELISA. The expression of TREM-1 mRNA of lung tissue was detected by RT-PCR. Results Compared with group A, the extent of lung damage was severer and it lasted a growing trend in group B, the water content of lung tissue in group B was increased. Simultaneously, the levels of TNF- α, IL-1 β, HMGB-1 and the expression of TREM-1 mRNA of lung tissue in group B were significantly higher, and the levels of TREM-1 mRNA and HMGB-1 were heightened duratively（P〈0.05）. Conclusion The levels of TREM-1 mRNA and HMGB-1 are positively correlated with the severity of severe acute pancreatitis associated lung injury.