BRCA1基因在白血病中的表达及其临床意义

Expression and significance of breast cancer suscepterbility gene 1 in leukemia

目的研究白血病患者的乳腺癌易感基因1（BRCA1）的表达水平及临床意义。方法采用荧光定量反转录．聚合酶链（RT-PCR）方法检测急性淋巴细胞白血病（ALL-L2）患者18例（初治13例，复发5例）、慢性粒细胞白血病（CML）慢性期患者20例、健康对照15名中的BRCA1的表达。结果初治ALL患者13例中，BRCA1 mRNA表达减低或缺失6例（46．1％），初治ALL组表达水平低于健康对照组（P〈0．05）；ALL完全缓解9例中，BRCA1 mRNA表达减低3例（33．3％），ALL完全缓解患者表达水平高于初治ALL组（P〈0．05），与健康对照组差异无统计学意义（P〉0．05）；ALL复发组5例，BRCA1 mRNA表达全部减低或缺失，ALL复发组表达水平低于健康对照组（P〈0．05），与初治ALL组差异无统计学意义（P〉0．05）。CML患者BRCA1mRNA表达水平与健康对照组比较差异无统计学意义（均P〉0．05）。结论BRCA1在不同类型白血病中的表达不同，提示其与白血病的预后密切相关，并可指导临床诊断和治疗。

Objective To investigate the expression and significance of breast cancer suscepterbility gene 1 （BRCA1） in leukemia. Methods Fluorescent quantitative reverse transcription-polymerase PCR was used to investigate the expression of BRCA1 in 18 patients with ALL-L2 （13 denovo ALL patients, 5 relapsed ALL patients）, 20 patients with CML-CP and 15 normal controls. Results The mRNA expression of BRCA1 in denovo patients with ALL was lower than that in normal control, with statistical significance （P 〈 0.05）; The mRNA level of BRCA1 in ALL patients in CR was higher compared with before to cure, with statistical significance （P 〈 0.05）, but lower than that in normal control, without statistical significance（P 〉 0.05）. The mRNA expression of BRCA1 in patients with relapsed ALL was lower than that in normal control with statistical significance （P 〈 0.05）, and lower than that in denovo patients with ALL, without statistical significance （P 〉 0.05）. The mRNA level of BRCA1 showed no difference in CML-CP patients compared with normal control （P 〉 0.05）. Conclusion The different expression of BRCA1 in leukemia indicates that he has closely relationship with the prognosis of leukemia and guides the clinical diagnosis and treatment.