BTW5对体外培养日本血吸虫成虫及童虫的杀灭效果

Schistosomicidal effect of BTW5 on adult and juvenile Schistosoma japonicum in vitro

目的观察BTW5对体外培养的日本血吸虫成虫和童虫的杀灭效果。方法小鼠感染日本血吸虫尾蚴18 d后用肝门静脉灌注法收集童虫,感染5周后用同法收集雌、雄成虫,分别体外培养于DMEM培养液中,加入不同浓度BTW5连续培养3 d,观察虫体死亡及活力降低情况,并设空白对照组。对虫体进行盐酸卡红染色,观察其损伤情况。结果在含不同浓度BTW5的DMEM培养液中,日本血吸虫成虫及童虫的死亡率与活力降低率均显著高于对照组(P均<0.05)。盐酸卡红染色直观显示BTW5对雌雄虫的体表和肠管有损伤作用,另外对雌虫的卵巢也有损伤。结论BTW5具有体外杀灭日本血吸虫成虫和童虫的作用。

Objective To evaluate the killing effect of BTW5 on both juvenile and adult worms of Schistosoma japonicum in vitro. Methods The mice were infected with cercariae of Schistosoma japonicum obtained from infected Oncomelania hupensis. The juvenile worms were obtained from the liver and mesenteric veins of the mice by perfusion 18 d after the infection, and the adult worms were obtained from the liver and mesenteric veins of the mice by perfusion 5 weeks after the infection. The adult and juvenile worms were cultivated in DMEM medium containing different concentrations of BTW5 and were observed for 3 d. After the observation, the worms were stained by hydrochloric acid carmine to observe the injuries. Results The death rates and motility reducing rates of adult and juvenile worms in DMEM medium containing different concentrations of BTW5 were significantly high-er than those in the controls. The staining suggested that BTW5 had a damage effect on the tegument and the intestinal canal of the male and female worms, in addition to the ovaries of the female worms. Conclusion BTW5 has an antischistosomal effect on adult and juvenile worms of Oncomelania hupensis.