广西2000～2010年O139群霍乱弧菌毒力检测及耐药性分析

Resistance and virulence gene of Vibrio cholera O139 in Guangxi in 2000～2010

目的研究广西O139群霍乱弧菌毒力基因携带情况及其药物敏感性。方法采用PCR方法检测广西2000~2010年分离到的14株O139群霍乱弧菌霍乱肠毒素（ctxA）毒力基因,同时用K-B法对10种推荐用抗生素氟哌酸（NOR）、庆大霉素（GEN）、复方新诺明（SXT）、四环素（TET）、氨苄青霉素（AMP）、阿米卡星（AMI）、环丙沙星（CIP）、强力霉素（DOX）、氯霉素（CHL）和链霉素（STR）进行耐药性检测。结果64.29%的菌株携带ctxA毒力基因,其中病人株携带ctxA毒力基因为100%;所有菌株对氟哌酸、阿米卡星和环丙沙星敏感,对其余7种抗生素高度耐药;病人及病家环境菌株的耐药性高于外环境菌株。结论庆大霉素、复方新诺明、四环素、氨苄青霉素、强力霉素、氯霉素和链霉素7种传统药物已不宜用于临床病人治疗,提示在疫情监测中应关注试验检测结果,指导临床用药,及时做好防控工作。

Objective To investigate the virulence gene and the antimicrobial susceptibility of Vibrio cholera O139 in Guangxi.Methods The method of PCR was applied to detect the virulence gene of cholera enterotoxin（ctxA） in 14 strains of Vibrio cholera O139 isolated from 2000 to 2010 in Guangxi,and the assay of K-B was applied to test the antimicrobial susceptibility of 10 kinds of antibacterial.Results Virulence gene was detected in 64.29% of strains Vibrio cholera serogroup O139,and in 100% of strains isolated from the patients.Antimicrobial susceptibility tests showed that all of the strains were susceptible to norfloxacin,aminkacin and ciprofloxacin,and were resistant to the other 7 categories of antibiotics.The strains isolated from patients and environment of the patients’families were more resistant to antibiotics than the strains isolated from the environment outside.Conclusions The conventional medications,Gentamycin,SXT,tetracycline,ampicillin,doxycycline,chloramphenicol and streptomycin,are not suitable for clinical treatment any more.It is suggested that the results of laboratory tests should be taken into account in epidemic surveillance to guide clinical treatment and disease control.