不同磁场环境和培养基对拟南芥种子诱导愈伤组织的影响

EFFECTS OF VARIOUS MAGNETIC FIELDS AND CULTURE MEDIUMS ON CALLUS INDUCTION FROM Arabidopsis SEEDS

为探明磁场强度和培养基成份在拟南芥愈伤组织诱导中的作用,以野生型拟南芥(Columbia型)种子为材料,研究3种磁场环境(亚磁场、地磁场和中强磁场)、4种诱导培养基(NB1、NB2、MS1、MS2)条件下愈伤组织的诱导情况。结果显示,与地磁场相比,亚磁场对接种后第3～6天愈伤组织的发生有明显的抑制作用,中强磁场有明显的促进作用;在接种后第3～7天,NB2、MS2比NB1、MS1培养基促进了愈伤组织形成,且差异显著。愈伤组织净重反映了细胞增殖的状态,统计结果显示,与地磁场相比,亚磁场对细胞增殖没有明显影响,而中强磁场有显著的抑制作用;NB2、MS2比NB1、MS1培养基促进了细胞增殖,且差异显著。以上试验结果说明,磁场强度和培养基成份的变化对拟南芥种子诱导愈伤组织过程中第3～7天的愈伤组织形成和细胞增殖有不同程度影响,但2因素不存在交互作用,且2种因素对15d后的愈伤组织总出愈率无显著影响。6-BA对早期的愈伤组织诱导和细胞增殖均有促进作用,而且在地磁和亚磁场中,添加有6-BA的NB2和MS2培养基所诱导的愈伤组织色泽鲜亮,致密度适中,利于后续继代培养。

Three magnetic fields（hypomagnetic fields,HMF;geomagnetic fields,GMF;moderate static magnetic fields,MSMF） and four culture mediums（NB1,NB2,MS1 and MS2） were applied to investigate the effects of magnetic field intensity and culture medium ingredient on callus induction in Arabidopsis（Columbia type）.The results indicated that magnetic intensity and culture medium ingredient had different effects on callus induction in whole process.In the first 3~6 days after inoculation,the callus induction rate under HMF was inhibited,but the rate under MSMF was promoted significantly contrasting with the GMF condition.As far as the culture medium was concerned,in the first 3~7 days after inoculation,the callus induction rate on NB2 and MS2 medium were significantly higher than that on NB1 and MS1.Callus net weight reflects the state of cell proliferation in some extent.Compared to the GMF condition,there was no effect on cell proliferation under HMF condition,while the inhibition effect under MSMF was significant.Cell proliferation on NB2 and MS2 medium was increased more significantly than that on NB1 and MS1.All of these results showed that the callus induction rate in early stage and cell proliferation were affected significantly by magnetic field intensity and medium composition.However,there was no effect on the total callus induction rate after 15 days of inoculation,and no interaction between the two factors.Under HMF and GMF conditions,the callus status on NB2 and MS2,added 6-BA showed bright color,moderate density and useful for the subsequent subculture.