葛根素对脉络膜血管内皮细胞增殖的影响

Effect of Puerarin on the proliferation of choroidal vascular endothelial cells in vitro

目的:观察不同浓度葛根素(Puerarin,Pur)对体外培养脉络膜血管内皮细胞(Choroidal vascular endothelial cells,CVECs)增殖的影响。方法:取第3代人CVECs用于实验。实验组中加入含不同浓度的Pur培养液,细胞对照组加入等量空白培养液。24 h及48 h后采用四甲基偶氮唑蓝比色法检测血管内皮细胞的增殖情况。结果:与细胞对照组比较,0.05、0.50、5.00μg/ml和50μg/ml Pur组作用24 h及48 h后CVECs A值均增加(P<0.01)。结论:Pur对体外培养的CVECs有明显的促进增殖作用,其作用与Pur的剂量有关。

Objective：To investigate the effect of different concentrations of Puerarin（Pur）on the proliferation of choroidal vascular endothelial cells（CVECs）in vitro.Methods：The third generation of CVECs were used for the research.The CVECs in experimental group were exposed to different concentrations of Pur while those in cell control group were exposed to equal amount of blank medium.The effect of Pur on the proliferation of cells was measured by methyl thiazolyl thiazolium（MTT）assay.Results：CVECs cultured with 0.05,0.50,5.00 μg/ml and 50.00 μg/ml Pur showed increase in absorbance（A）value at 24 h and 48 h after treatment（P〈0.01）.Conclusion：Pur can obvious promote the proliferation of CVECs,which is related with the dosage of Pur.