摘要
目的分析2008—2011年南通市麻疹流行病学流行特征,为控制麻疹的发病,制订消除麻疹策略提供科学依据。方法采用描述性流行病学方法对麻疹监测系统资料进行分析,同时用Vero-Slam细胞分离麻疹病毒。对分离得到的麻疹病毒,用RT-PCR法扩增N基因的C末端,测定其核苷酸序列,并与WHO的参照毒株序列比较,构建基因亲缘关系树,确定南通市麻疹野毒株的基因型;同时对南通MV野毒株N基因C末端进行核苷酸同源性分析。结果 2008—2011年南通市麻疹的发病率分别为3.73/10万、2.55/10万、0.45/10万和0.36/10万,呈逐年下降趋势,基因分型结果表明,南通MV野毒株均为H1型。结论强化高危人群的免疫,建立监测体系是控制麻疹积极有效的手段。
OBJECTIVE In order to provide scientific basis for measles elimination, the epidemiological characteristics and the current prevalence strain of wild measles virus were studied in Nantong City, during 2008--2011. METHODS Descriptive epidemiology was used to analyze the＇ data from measles reporting and sttrveillance system. Virus transport medium from measles IgM positive cases were inoculated onto Veto/SLAM cells to isolate the measles virus. The isolated strains were phylogenetic analyzed according the nucleotide sequencing of the c-terminal region of the nuc]eoprotein gene amplified by RT-PCR. RESULTS The incidence of measles in Nantong City during 2008--2011 were 3.73/100 000, 2.55/100 000, 0.45/100 000 and 0.36/100 000 respectively. RESULTS Showed that measles confirmed cases in Nantong City declined year by year. All of 16 isolates were clustered within genotype H1, the same as the dominant genotype prevalence in China. CONCLUSION The strengthening immunity in high-risk groups, the monitoring and control system building should be the effective means to eliminate measles.
出处
《中国初级卫生保健》
2012年第10期79-80,共2页
Chinese Primary Health Care
关键词
麻疹
流行病学
监测
基因型
measles
epidemiology
monitoring system
gene type
