白细胞介素-4在油酸性急性肺损伤家兔的表达

Expression of interleukin-4 in rabbits with acute lung injury induced by oleic acid

目的观察白细胞介素-4(IL-4)在油酸性急性肺损伤(ALI)家兔的表达,探讨IL-4在家兔油酸性ALI发病机制中的作用。方法 20只新西兰家兔随机分为油酸性ALI模型组(实验组)和对照组,每组10只。检测2组家兔动脉血氧分压(PaO2)、肺组织IL-4表达情况和髓过氧化物酶(MPO)活性、外周血和支气管肺泡灌洗液(BALF)中IL-4表达水平,并进行比较。结果实验组家兔肺组织、外周血和BALF中IL-4的表达水平显著高于对照组(P<0.05),实验组家兔PaO2显著低于对照组(P<0.05);实验组家兔肺组织中MPO活性显著高于对照组(P<0.01);实验组家兔PaO2水平与IL-4的表达呈负相关(r=-0.412 5,P<0.05)。结论 IL-4表达上调与家兔ALI的发生发展有关。

Objective To observe the expression of interleukin-4（IL-4） in rabbits with acute lung injury（ALI） induced by oleic acid,in order to explore the effect of IL-4 in the pathogenesy of rabbits with ALI induced by oleic acid.Methods Twenty New Zealand rabbits were randomly divided into ALI model group（experiment group） and control group,ten rabbits in each group.The partial pressure of oxygen in artery（PaO2）,the expression of IL-4 and the activity of myeloperoxidase（MPO）,the levels of IL-4 in peripheral blood and bronchial alveolar lavage fluid（BALF） were detected and compared in two groups.Results The levels of IL-4 in lung tissue,peripheral blood and BALF of rabbits in the experiment group were significantly higher than those in the control group（P0.05）.The PaO2 in the experiment group was significantly lower than that in the control group（P0.05）.The activity of MPO in the experiment group was significantly higher than that in the control group（P0.01）.The level of PaO2 was negatively correlated to the expression of IL-4 in the experiment group（r=-0.412 5,P0.05）.Conclusion The increasing of the expression of IL-4 may be associated with the development of ALI in rabbits.