FISH技术在鉴别上尿路尿道上皮癌与良性疾病引起血尿中的作用

Roles of fluorescence in situ hybridization in differential diagnosis of urothelial carcinoma in upper urinary tract from benign diseases with hematuria

目的探讨荧光原位杂交技术(fluorescence in situ hybridization,FISH)作为非创性分子检查方法,用于鉴别上尿路尿道上皮癌(upper urinary tract urothelial carcinoma,UUT-UC)和良性泌尿系统疾病引起的血尿。方法运用一组荧光标记的DNA探针检测34例UUT-UC患者和33例泌尿系统良性疾病患者血尿脱落细胞中3、7、9、17号染色体异常,并对结果进行分析。结果 34例UUT-UC患者血尿中25例(73.5%)检测出染色体异常,而良性疾病患者血尿脱落细胞检测出2例发生染色体异常(6.1%)(P<0.001)。结论 FISH检测脱落细胞3、7、9、17染色体异常,可以作为一种非创性方法用于鉴别UUT-UC和良性疾病引起的血尿。

Purpose To evaluate the clinical utility of a fluorescence in situ hybridization（FISH） assay as a non-invasively molecular test to distinguish urothelial carcinoma in upper urinary tract（UUT-UC） from benign lesions presenting with hematuria.Methods The chromosomal abnormalities of chromosomes 3,7,17,and 9（p16） in hematuria specimens from 34 patients with UUT-UC and 33 patients with benign disorders were detected by using a set of fluorescently labeled DNA probes.The abnormalities of chromosomes were determined and analyzed between UUT-UC and benign disorders.Results The abnormalities of chromosomes were detected in 25 out of 34（73.5%） patients with UUT UC and in 2 out of 33（6.1%） patients with benign disorders（P0.001）.Conclusions FISH assay of chromosomes 3,7,9,and 17 performed on exfoliated cells from voided urine specimens could be used as a non-invasive method to distinguish urothelial carcinoma in upper urinary tract from benign disorders presenting with hematuria.