摘要
目的探讨荧光原位杂交(fluorescence in situ hybridization,FISH)与免疫组织化学(immunohistochemistry,IHC)检测胃癌组织中HER-2基因扩增及与C-erbB-2蛋白表达结果的一致性。方法采用FISH和IHC法分别检测50例胃癌组织中HER-2基因扩增和C-erbB-2蛋白表达,并采用SPSS 19.0进行统计学分析。结果 50例胃癌标本进行IHC检测结果 11例为(﹢),9例为(),4例为(),26例为(-);50例胃癌标本进行FISH检测结果 11例为(+),39例为(-);其中,FISH检测阳性标本中IHC检测有2例(﹢),5例(),4例()。IHC检测C-erbB-2蛋白为()的病例FISH检测均为(+),而IHC检测C-erbB-2蛋白()的9位患者中有4例经FISH检测证实HER-2呈(-),5例呈(+)。结论 IHC与FISH检测HER-2状态结果的符合率为95.1%(39/41),具有高度一致性(Kappa系数=0.773,P<0.001)。当IHC检测C-erbB-2蛋白表达为(-)或()时IHC和FISH检测结果有较高符合率,可作为临床是否应用Herceptin治疗的依据,而C-erbB-2蛋白表达为()的病例则必须进一步行HER-2基因扩增检测。
Purpose To investigate the consistency of HER-2 expression in gastric carcinoma by fluorescence in situ hybridization（FISH） and immunohistochemistry（IHC）.Methods The expression of C-erbB-2 protein and HER-2 gene amplification in 50 cases of gastric cancer were detected by IHC and FISH,respectively,and the concordance between them was analyzed by SPSS 19.0.Results The expression of C-erbB-2 protein was negative,weak,moderately and strongly positive in 26,11,9 and 4 cases,respectively,by IHC.11 cases were HER-2 gene positive in FISH,among which 2,5 and 4 cases were weak,moderately and strongly positive in IHC respectively.All of 4 cases with strongly positive C-erbB-2 expression by IHC were positive in FISH.However,HER-2 gene amplification existed in 5 cases among 9 cases with moderately positive C-erbB-2 expression by IHC.Conclusions The concordance rate in our investigation between IHC and FISH of HER-2 is 95.1%,with a high degree of consistency（Kappa coefficient 0.773,P0.001）.In addition,the results of IHC and FISH have a higher coincidence rate in the cases with strongly positive or negative（negative or weak positive） expression of C-erbB-2.It can be an indication to decide whether herceptin can be used in clinical therapy or not.But in the cases with moderately positive expression of C-erbB-2,the results of FISH are diversified,indicating these cases need further detection by FISH.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第10期1094-1097,共4页
Chinese Journal of Clinical and Experimental Pathology
