阳离子对茶叶几种酶的作用机理研究

Effect of cations on activity of some enzymes in tea shoot

以秋梢鲜叶为材料 ,测定了多酚氧化酶、过氧化物酶和谷氨酸脱氢酶的活性以及脱辅基并用阳离子处理后的酶活性。结果表明 ,阳离子处理后 ,三种酶活性较对应的脱辅基酶活性均得到不同程度的提高 ;与脱辅基前活性相比却因阳离子的不同而异。即使是同一种阳离子也因浓度的不同而不同。据此可判断。阳离子对茶叶中酶的作用机理是作为酶的辅基或激活剂 ,并在茶树体内向幼嫩部位分布。

Activity of polyphenolic oxidase,peroxidase and glutamic dehydrogenase in autumn tea shoots as well as the activity of these enzymes which were without subsidiary groups and treated with cations were determined.The results showed that the activity of the three enzymes whose subsidiary groups were removed was increased by cations treatment and their activity was influenced by the type of cations and the cation’s concentration.It suggested that cations acted as subsidiary group or activator of enzymes in tea leaf and they moved to the tender shoots in the tea plant. [WT5”HZ]