人前列腺癌裸鼠皮下移植瘤模型的建立

Establishment of Subcutaneous Xenograft Model of Human Prostate Carcinoma in Nude Mice

目的建立人前列腺癌裸鼠皮下移植瘤模型。方法采用人前列腺癌细胞株PC-3细胞接种于裸鼠的颈背部皮下,计算成瘤潜伏期、成瘤百分率,观察移植瘤大体生长情况,绘制生长曲线,并对移植瘤进行病理鉴定。结果采用人前列腺癌细胞株PC-3细胞皮下接种方式建立移植瘤的平均成瘤潜伏期为24天,成瘤百分率为100%,瘤体积倍增时间为10天左右,移植瘤的形态和功能特性与原发肿瘤基本相似。结论本动物模型的建立可为前列腺癌的放射免疫显像以及放射免疫治疗提供一个有价值的实验平台。

Objective To establish a subcutaneous xenograft model of human prostate carcinoma in nude mice.Methods PC-3 cells of the human prostate carcinoma were inoculated subcutaneously into the neck back of BAlB/c nude mice.The tumor incubation period,forming tumor percentage was calculated and pathological identification of tumors were carried out Results The mean incubation period of xenograft by inoculating subcutaneously with human prostate PC-3 cells was 24 days with 100% tumor forming.The time of tumor volume doubled was 10 days.Morphological and functional features of xenograft were basically same as original tumor.Conclusion The xenograft model of human prostate carcinoma established in this study could provide valuable experiment platform for radioimmunoimaging and radioimmunotherapy of the prostate carcinoma.