冠心病患者血小板CD31及血浆纤维蛋白原表达水平的测定及其意义

Expression level and clinical significance of platelet CD31 and plasma fibrinogen in patients with coronary heart disease

目的检测冠心病(CHD)患者血小板内皮细胞黏附分子1(CD31)及血浆纤维蛋白原(FIB)水平,分析其与CHD严重程度的相关性并探讨其临床意义。方法入选研究对象97例,分为稳定型心绞痛(SAP)组(21例),急性冠脉综合征(ACS)组(43例)和正常对照组(33例)。采用流式细胞术检测血小板CD31,凝固法测定血浆FIB的表达水平。结果 ACS组血小板表面活性标志物CD31[(93.85±14.84)%],血浆FIB水平[(3.66±0.91)g/L]均明显高于对照组[(78.69±27.09)%,(3.06±0.62)g/L]和SAP组[(82.80±28.00)%,(3.14±0.56)g/L(均为P<0.05)];而对照组与SAP组CD31、FIB表达水平差异无统计学意义(均为P>0.05)。相关分析显示CD31与FIB呈显著正相关(r=0.337,P<0.01)。结论检测CHD患者CD31、FIB水平,能较好地反映CHD严重程度,对临床有重要的应用价值。

Objective To study the expression level and clinical significance of platelet endothelial cells adhesion molecule-1（CD31） and plasma fibrinogen（FIB） in patients with coronary heart disease（CHD）. Methods A total of 97 patients,including 21 patients with stable angina pectoris（SAP group）,43 with acute coronary syndrome（ACS group） and 33 controls（control group）were studied.The expression level of platelet CD31 was measured by flow cytometry and plasma FIB was measured by coagulation method. Results The expression level of platelet CD31 [（93.85±14.84）%] and plasma FIB [（3.66±0.91）g/L] in ACS group were significantly higher than in control group [（78.69 ±27.09）% and（3.06±0.62）g/L）] and SAP group [（82.80±28.00）% and（3.14±0.56）g/L]（all P0.05）.There were no significant differences in platelet CD31 and plasma FIB between control group and SAP group（P0.05）.Correlation analysis demonstrated that platelet CD31 was positively correlated with plasma FIB（r=0.337,P0.01）. Conclusions The expression level of platelet CD31 and plasma FIB in CHD patients can reflect the severity of disease and has important clinical values.