TREM-1基因在重症急性胰腺炎大鼠中的表达及意义

Expression and significance of TREM-1 gene in mice with severe acute pancreatitis

目的通过TREM-1基因在重症急性胰腺炎大鼠胰腺、肺组织中的基因表达，探讨TREM。1基因在重症急性胰腺炎发病机制中的作用，进而为治疗重症急性胰腺炎提供新的分子靶点。方法健康雄性Wistar大鼠30只，完全随机分为两组，每组15只。A组：正常组；B组：重症急性胰腺炎组；用5％牛黄胆酸钠逆行胰胆管注射制作重症急性胰腺炎模型。结果TREM-1相对表达量比较：Real—time PCR进行相对定量检测大鼠胰腺、肺组织中TREM-1的相对表达量，B组胰腺、肺两个组织中TREM-1 mRNA相对含量的在6h、12h、24h相应时间点相比明显高于A组，差异有显著性统计学意义（P〈0．05），且随时间变化逐渐升高；且与胰腺病理变化呈正相关。结论重症急性胰腺炎时，TREM-1的相对表达量在大鼠胰腺、肺组织中的明显表达，更能加重胰腺损伤及全身炎症反应综合征。

Objective Through detecting the expression of triggering receptor expressed on myeloid cells-1 （TREM- 1 ） in pancreas and lung of rats with severe acute pancreatitis, to approach the role of TREM- 1 in severe actute pancreatitis pathogenesis, and provid a new molecular target for SAP. Methods Thirty healthy male Wistar rat were randomly divided into two groups： control group（ group A） , n = 15 ;severe acute pancreatitis group （group B） , n = 15. The model of severe acute pancreatitis of rats was induced by retrograde injection of 5 % sodium taurocholate into the pancreatic duct. Results TREM- 1 in pancreatic tissues and lung tissues was significantly higher in group B than group A in three time points （ 6 h, 12 h, 24 h） and had significant changes （ P 〈 0.05 ）, and had a positive correlation with pancreatic pathology. Conclusion TREM-1 was significantly expressed in severe acute pancreatitis, and aggravated pancreas damage and systemic inflammatory response syndrome.