椎痛消胶囊对腰椎间盘突出症大鼠炎性细胞的影响

Effect of Zhuitongxiao Capsule on inflammatory cells of rats with lumbar disc herniation

目的通过椎痛消胶囊药效学实验,评价炎性细胞在腰椎间盘突出症中的作用及椎痛消胶囊对其的影响。方法将健康雄性Wistar大鼠60只随机分为椎痛消胶囊高、中、低剂量组及阳性对照组、模型对照组、正常对照组,每组10只。采用自体髓核植入,模拟腰椎间盘突出症模型。各组连续给药30d,正常对照组、模型对照组灌服等量蒸馏水。对各组大鼠的背根神经节、神经根进行组织学观察,计数炎性细胞。结果模型组神经节、神经根均出现明显的炎性细胞漫润;椎痛消胶囊高、中、低剂量组及阳性对照组的背根神经节、神经根术后的炎性细胞漫润数目明显减少,与模型组相比差异有统计学意义(P<0.05,P<0.01,P<0.001),相应的神经根损害明显减轻。结论髓核组织介导的炎性细胞浸润及炎症反应在腰椎间盘突出症中起重要作用;椎痛消胶囊能有效减少炎性细胞浸润、减轻炎症反应和神经组织的损伤,对促进突出物的吸收有重要意义。

Objective To evaluate the effect of inflammatory cells in lumbar disc herniation and the influence of ZhuiTongxiao Capsule through pharmacodynamics experiment. Method Divide randomly the 60 healthy male Wistar rats into six groups, namely, high, middle and low close groups of ZhuiTongxiao Capsule, positive con- trol group, model control group and normal control group, with 10 rats in each group. Implant autologous nu- cleus pulposus to simulate lumbar disc herniation. All the groups take drugs for successive 30 days, and the normal and model control group take equivalent mount of distilled water. Do histological observation for dorsal root ganglions and nerve roots and count the number of inflammatory cells. Results The ganglions and nerve roots in model group appeared obvious inflammatory cell infiltration. The number of postoperative inflammatory cell infiltration of dorsal root ganglion and nerve root number decreased obviously in high, middle and low dose groups and positive control group. Compared with the model group, the difference had statistical significance （P 〈0. 05, P 〈0. 01, P 〈0. 001 ） . The corresponding nerve root damage reduced obviously. Conclusions Inflammatory cell infiltration and inflammatory reaction in nucleus pulposus tissue mediate played important roles in lumbar disc herniation. ZhuiTongxiao Capsule could effectively reduce inflammatory cells infiltration, alleviate inflammatory reation and nerve tissue damage, which had important significance for promoting the ab- sorption of projection.