沙棘微繁育苗产业化试验研究——水培沙棘茎段插穗不定根发生的机理

Experimental Research on Seabuckthorn Seedlings Industrialization with Micropropagation ——Hydroponic seabuckthorn short stem section of Cuttings adventitious roots occur

本文用水培方法研究了沙棘短茎段插穗生根的机理。结果表明,在用外源激素处理、控温控湿条件下,沙棘可以用极短的茎段(1～3芽段)插穗产生不定根,进而形成新的植物。沙棘插穗不定根形成有两种方式:一是茎段切面先产生愈伤组织,然后再分化为不定根;二是直接由茎皮层内分生组织产生不定根;且不定根产生具"茎多部位生根效应"。沙棘插穗不定根形成属于"温度敏感型",最适温度为16℃～26℃。外源激素对沙棘短茎段插穗生根表现出良好的诱导效应,而且对插穗苗的生长发育一定的影响,ABT优于GGR;"壮根素"效果优于生根粉,不仅促进发根,而且促进成熟不定根的早期结瘤。

The rooting mechanism of seabuckthorn short stem section for micropropagation with aqua- culture was studied. The results show that short stem section （1-3 buds） of seabuckthorn cuttings can produce adventitious roots, form forward a new small plant under the exogenous hormones and controlling temperature and moisture. The adventitious roots formation of seabuckthorn cuttings have two different ways, first way is cutting producing callus and then subdividing into adventitious roots, second way is the cortex meristematic tissue producing directly adventitious roots, and adventitious roots producing with ＂ more parts stem rooting effect＂ . The adventitious roots formation of sea-buckthorns seedlings belongs to ＂ temperature sensitive type＂, the optimal temperature is 16℃～26℃. Different exogenous hormones promote seabuckthorn cuttings rooting and affect the seedlings growth and development, ABT is superior to GGR. ＂Strong root element＂ is more than rooting powders. Strong root element has the function of producing nodular factor and seedlings growth and development.