粪便肠脱落细胞端粒酶活性检测对大肠癌的早期诊断价值

Value of detecting Telomerase activity in colorectal exfoliated cell for early diagnosis of colorectal cancer

目的探讨粪便肠脱落细胞、组织细胞中端粒酶活性表达对大肠癌早期诊断的意义。方法端粒酶PCR-ELISA检测法检测30例大肠癌患者、30例大肠腺瘤患者及30例对照组健康自愿者粪便肠脱落细胞及对应组织细胞标本中端粒酶活性表达。结果大肠癌组粪便肠脱落细胞标本中,端粒酶阳性表达率为83.3%(25/30)。大肠腺瘤组粪便肠脱落细胞标本中,端粒酶阳性表达率为66.6%(20/30)。对照组粪便肠脱落细胞中端粒酶阳性表达率为3.3%(1/30)。27例大肠癌组织端粒酶表达阳性的患者有25例其对应的粪便肠脱落细胞端粒酶活性也呈阳性表达。结论粪便肠脱落细胞端粒酶活性表达对大肠癌的早期诊断具有重要意义。

Objective To investigate the value of detecting Telomerase activity in colorectal exfoliated cell and tissue cell for early diagnosis of colorectal cancer.Methods We detected Telomerase activity by PCR-ELISA in fecal DNA and tissues DNA from 30 colorectal cancer patients versus 30 samples from adenoma patients and 30 healthy subjects.Results The positive rate of Telomerase activity detection in exfoliated cell in stool were 83.3%（25/30）,66.6%（20/30） and 3.3%（1/30） of patients with colorectal cancer,adenoma and healthy subjects,respectively.Among 27 cases which were detected positive in tumor tissues,25 cases were also positive in exfoliated cell in stool.Conclusion Detection of Telomerase activity in stool has important significance in early diagnosis of colorectal cancer.