乙酰肝素酶、组织蛋白酶D在宫颈癌的表达

Expression of heparanase and cathepsin D in cervical cancer

目的探讨乙酰肝素酶(Hps)和组织蛋白酶D(CathD)与宫颈癌的发生、发展及预后的关系。方法取我院宫颈癌住院手术患者组织标本89例;宫颈上皮内瘤变(CIN)41例,正常宫颈组织35例。采用免疫组化SP法对乙酰肝素酶和组织蛋白酶D的表达进行检测。结果宫颈癌组织中乙酰肝素酶和组织蛋白酶D的免疫积分明显高于宫颈CIN组织及正常宫颈组织。而在CIN和正常宫颈组织间比较无统计学差异。乙酰肝素酶和组织蛋白酶D的表达与宫颈癌的临床分期、血管浸润和淋巴结转移有关。结论乙酰肝素酶和组织蛋白酶D可能在宫颈癌的发生、发展及预后中起着重要作用。

Objective To analyze the relationship between heparanase,cathepsin D and the occurrence,development and prognosis of cervical cancer.Methods Specimens were obtained from 89 patients with cervical carcinoma,41 patients with cervical intraepithelial neoplasia（CIN） and 35 patients with normal cervix in our hospital.SP immunochistochemistry technique was used to detect the expressions of heparanase,cathepsin D in tissues.Results The staining score of heparanase and cathepsin D in cervical carcinoma was higher than in CIN and normal cervix.There was no statistical difference of heparanase and cathepsin D expression between in CIN and normal cervix.A significant correlation was found between the expression of heparanase and cathepsin D and clinical stage,vascular invasion and lymphatic metastasis of cervical carcinoma.Conclusion Heparanase and cathepsin D maybe play an important role in the occurrence,development and prognosis of cervical cancer.