上海地区不同表型香樟的遗传多样性分析

Analysis of Genetic Diversity of Different Phenotypes Cinnamomum camphora in Shanghai

探索香樟的遗传多样性,为园林绿化提供理论依据,本研究利用RAPD分子标记技术,对上海地区5种不同表型的香樟样品进行了遗传多样性分析,从61条随机引物中筛选出7条多态性引物进行PCR扩增,共扩增出72条DNA带,平均每个引物检测到条带为10.29条。结果表明,5个表型中多态比率最大的是表型B,为68.06%;最低的是表型E,为44.44%。各表型间的遗传相似系数介于0.916～0.980之间,表明上海地区香樟各表型间的遗传多样性较低。

To explore the genetic diversity and offer theory supports for garden virescence, the genetic diversity of five kinds of different phenotypes Cinnamornurn camphora in Shanghai was analyzed using RAPD. 7 excellent random primers for PCR amplification polymorphism were screened from 61 random primers,and 72 bands were obtained, average 10. 29 bands had been detected hy each random primer. Among five kinds of different phenotypes, phenotype B had the highest polymorphic loci ratio, which was 68. 06% by contrast, the lowest polymorphic loci ratio belonged to phenotype F, which was 44. 44%. According to the results, the genetic similarity coefficient varied from 0. 916 to 0. 980, this indicated that the genetic diversity among five kinds of different phenotypes Cinnamomum camphora was lower.