小麦基因组DNA提取方法比较研究

Study on comparison of methods of extracting genome DNA from wheat

用4种方法对小麦基因组DNA进行提取,采用琼脂糖凝胶电泳法和核酸蛋白分析仪比较DNA的浓度和质量,结果显示,4种DNA提取方法在DNA浓度上有所差别,方法一所提取的DNA浓度最高,但消耗试剂最多;方法二所提取的DNA浓度最低,有蛋白质污染,但过程简单;方法三和方法四所提取的DNA浓度相当,方法三有蛋白质污染,过程简单,方法四纯度高,过程最复杂。采用同一对引物和反应体系对所提取的DNA进行PCR扩增,结果显示4种方法所提取的基因组DNA均能满足分子标记检测的需要。

This paper introduces 4 methods of wheat DNA extraction. The DNA concentration and quality are compared by using agarose gel electrophoresis and protein nucteic acid analyzer. Experimental results show that there is somewhat difference in DNA concentration between the 4 methods. The DNA concentration in Program I is the highest, but the consumption of reagent is the most. The DNA concentration of Program Ⅱ is the lowest, but the process is simple. The extracted DNA concentrations are equivalent to Programs Ⅲ and Ⅳ. There is protein pollution in Program Ⅲ, but the process is simple. The purity of Program rv is higher than others, but the process is the most complex, Using the same pair of primers and the reaction system, the extracted DNA is amplified by PCR. The results show that the 4 methods all can meet the requirement of molecular marker detection.