支原体肺炎早期诊断中荧光定量PCR优于血清学试验

Fluorescent quantitation PCR was more excellent than serology for the early diagnosis of Mycoplasma pneumoniae pneumonia

目的探讨荧光定量PCR在早期肺炎支原体肺炎(Mpp)中的诊断价值。方法 2009年3月至2011年9月收治入院诊断为社区获得性肺炎患者,分别在入院第1、8、15天用荧光定量PCR及血清学方法检测肺炎支原体(Mp)。结果①226例社区获得性肺炎中肺炎支原体感染47例,检出率为20.8%。②入院第1天,荧光定量PCR诊断支原体肺炎41例,高于Mp-IgM诊断26例,差异有统计学意义(χ2=8.516,P<0.05)。③Mp感染年龄段主要在18～35岁。④与普通CAP相比Mp肺炎临床表现无特异性。结论①Mp是CAP主要的病原体之一。②Mp感染1周内,荧光定量PCR检测优于血清学试验。

Objective To explore the diagnostic value of fluorescent quantitation PCR for early Myco- plasma pneumoniae pneumonia. Methods A prospective study was performed on 226 adult CAP patients dur- ing 2 years and 6 months. Mycoplasma pneumoniae was tested by fluorescent quantitative PCR and by serologi- cal methods during the first, eighth and fifteenth day. Results （1）47 patients was diagnosed with Mycoplasma pneumoniae infection（ 20. 8% ）. （2）In the first day after admission, mycoplasma pneumoniae pneumonia was di- agnosised by fluorescent quantitation PCR（41 patients）, was more excellent than Mp-IgM （26patients）, （X2 = 8. 516, P 〈 0. 05 ）. （3）Mycoplasma pneumoniae infections were more frequent between patients aged 18-35 years. No significant difference of symptoms between the Mycoplasma pneumoniae pneumonia and common CAP. Conclusion Mycoplasma pneumoniae remains one of the most important pathogens. During the first week after disease onset the performance of fluorescent quantitation PCR was more excellent than Mp-IgM.