一种适用于初试者的STZ法建T1DM大鼠模型的技术方案

A new and suitable technical scheme of establishing type 1 diabetes (T1DM) rats model by STZ injection for beginners

目的:探索一种适用于初学者的以STZ法复制1型糖尿病(T1DM)大鼠模型的技术方案。方法:100只雄性Wistar大鼠随机分为模型组(DMM)和对照组(NC)。采用有别于现有文献报道的技术方案即分批次建模。DMM组分3批依次分别一次性腹腔注射60 mg/kg(DMM1)、70 mg/kg(DMM2)、55 mg/kg(DMM3)STZ溶液,NC组注射等量的柠檬酸盐缓冲液。DMM1、DMM2建模未成功者均编为DMM3。将72 h后禁食17 h空腹血糖值(FPG)≥11.1 mmol/L和相应生理指标作为成模的判断依据。结果:DMM1建模前后FPG无差异(P>0.05),DMM1建模失败;DMM2建模前后FPG具有显著差异(P<0.05),成模率为75%。DMM3补充剂量后建模成功。结论:分批次进行的STZ法建模方案适宜于初学者使用。

Objective：To explore a new and suitable technical scheme of establishing type 1 diabetes （T1DM） rats model by STZ injection for beginners. Methods：Total 100 male Wistar rats were divided into diabetes mellitus model （DMM） group and the control group （NC） randomly. The technical scheme for establishing animal model is different from the existing literature. The DMM group of two batches were followed by a single intraperitoneal injection 60 mg/kg （DMM1） or 70 mg/kg （DMM2） STZ solution respectively. the NC group was injected with the same amount of citrate buffer. The rats in DMM1 and DMM2 goups,which didn＇t develop T1DM, were compiled into DMM3 group （55 mg/kg STZ by a intraperitoneal injection）. After 72 h of STZ treatment,the rats were eonsiderd as TIDM model with fasting plasma glucose （FPG） after 17 h fasting ≥ 11.1 mmol /L and with corresponding physiological indicators. Results：The rat model can＇t be constructed successfully by a single intraperitoneal injection STZ of 60 mg/kg. The FPG of rats in DMM2 group changed significantly before and after STZ injection （P 〈 0.05）,and 75% of rats developed T1DM. The rats that didn＇t develop T1DM with first injection of STZ developed T1DM after supplementary dose in DMM3 group. Conclusion： Constructing T1DM rats induced by STZ injection in batch is best for beginners to use.