灰毡毛忍冬与忍冬的药理作用比较研究

Comparison of the pharmacological effects between L. macranthoides Hand. -Mazz and Lonicera japonica Thunb

目的对《中国药典》新增药材忍冬科植物灰毡毛忍冬与传统忍冬的主要药理作用进行比较研究。方法观察两种受试药物体外对金葡菌等6种常见致病菌的抗菌作用;通过建立小鼠毛细血管通透性增加、大鼠足肿胀、大鼠棉球肉芽增生的模型等试验评价药物抗炎作用;评价药物对环磷酰胺致免疫低下小鼠免疫器官重量、巨噬细胞吞噬功能的影响。结果灰毡毛忍冬和忍冬对6种常见致病菌均有明显地抑菌和杀菌作用,MIC分别在7.8～62.8 g·L-1和15.7～125.5 g·L-1,MBC分别≥250 g·L-1和≥500 g·L-1;二者剂量各为1 g·kg-1时,均能明显降低小鼠毛细血管通透性、抑制大鼠足肿胀度和肉芽组织的增生;明显提高小鼠脏器指数、廓清指数K和吞噬指数α,且两种受试药物药效强度差异无显著性。结论灰毡毛忍冬和忍冬均具有抗菌、抗炎的作用,均能增强动物非特异性免疫,且二者的作用强度相当。

Aim To study the main pharmacological effects of L. macranthoides Hand. -Mazz and Lonicera japonica Thunb.Methods Through observing the antibacterial activities on 6 kinds of bacteria in vitro,the antibacterial activities of the two kinds of medicinal materials were compared.The anti-inflammatory effects of them were compared by modeling increase of blood capillary permeability with mice,swelling of foot and granuloma on tampon with rats;the immunological regulation was detected by weighing the immune organs of low-immunity mice induced by injecting CTX,and the phagocytosis function of phagocytic cells in mice was researched.Results The antibacterial activities of L. macranthoides Hand. -Mazz and Lonicera japonica Thunb were different on those 6 kinds of bacteria.MIC：7.8～62.8 g·L-1 and 15.7～125 g·L-1,MBC： ≥250 g·L-1 and ≥500 g·L-1.They could obviously reduce the blood capillary permeability increase of mice,the foot swelling degree of rats and the hyperplasia of granuloma of rats.In addition,with the administration of them,the organ index,the clearance index K and the phagocytosis index α were increased.However,the effects of them showed no significant difference.Conclusions Both（L. -macranthoides Hand. -Mazz） and Lonicera japonica Thunb have antibacterial,anti-inflammatory and non-specific immunity functions.And these functions of them are almost equal.