正交分析法优选半乳糖化羧甲基壳聚糖磁性纳米载体的制备条件

Optimize the preparing condition of galactosylated-carboxymethyl chitosan-magnetic iron oxide nanoparticles by orthogonal method

目的:优选半乳糖化羧甲基壳聚糖磁性纳米载体(galactosylated-carboxymethyl chitosan-magnetic iron ox-ide nanoparticles,Gal-CMCS-Fe3O4NP)的制备条件。方法 :采用正交分析试验方法 ,以Fe3O4NP与羧甲基壳聚糖(car-boxymethyl chitosan,CMCS)质量比、pH值和搅拌时间为考察因素,纳米载体的粒径和磁响应性为考察指标。结果 :Fe3O4NP与CMCS的质量比为1∶2、pH值为7、搅拌时间为1 h时为最佳制备条件,最终所制备的Gal-CMCS-Fe3O4NP的平均粒径为20 nm,具有较好的磁响应性。结论:获得较满意的Gal-CMCS-Fe3O4NP的制备条件,纳米载体性状符合要求。

Objective： To optimize the preparing condition of galactosylated-carboxymethyl chitosan-magnetic iron oxide nanoparticles（Gal-CMCS-Fe3O4NP）.Methods： Orthogonal experiment was designed with the particle diameter and magnetic property as index,and with quality ratio between Fe3O4NP and carboxymethyl chitosan（CMCS）,pH and the stirring time as factors.Results： The orthogonal analysis indicated that the optimal preparation conditions was followed,quality ratio between Fe3O4NP and carboxymethyl chitosan（CMCS） was 1∶2,pH was 7 and the stirring time was 1 hour.The final Gal-CMCS-Fe3O4NP have good magnetic property,and the average particle diameter is 20 nm.Conclusion： With this method,a satisfactory preparing condition can be obtained,and the character of the nanoparticles is desirable.