我国1株鹦鹉幼稚病毒的细胞培养特性

Growth Characteristics of a New Strain of Psittacine Virus Found in China

为进一步研究鹦鹉幼稚病病毒 ( BFDV)的分子生物学特性奠定基础 ,对 BFDV湖北分离株进行了 2种原代细胞的适应性培养。首先在鹦鹉胚成纤维细胞盲传至第 5代后再过渡到鸡胚成纤维细胞培养 ,建立了BFDV的适应细胞培养体系。观察了 3种不同温度对细胞病变 ( CPE)的影响 ,结果表明 ,BFDV在 3 8.5℃培养 4 8～ 96h可产生明显的 CPE。再将该培养液做免疫扩散试验结果出现明显的免疫复合物沉淀线 ,抗体中和试验显示 CPE消失 ,进一步证实此 CPE是由 BFDV增殖而产生的。研究证明 。

A new strain of psittacine virus was isolated from fledgling budgerigars by our laberatory in 1995, and had been designated as Budgerigar fledgling disease virus(BFDV). The virus was cultured initially on budgerigar embryo fibroblast(BEF) for 5 generations, then cultured on chicken embryo fibroblast (CEF) for 13 generations. The tissue culture of BFDV showed the cytopathogenic effect (CPE) under 38.5℃ for 48 96 h.