角质细胞生长因子促进大鼠胰腺导管上皮细胞体外增殖

Keratinocyte growth factor promotes proliferation of rat pancreatic ductal epithelium cells in vitro

目的探讨不同浓度的角质细胞生长因子(KGF)对大鼠胰腺导管上皮细胞增殖的影响,寻求最佳浓度。方法免疫细胞化学染色及RT-PCR方法鉴定SD大鼠胰腺导管上皮细胞;不同浓度的KGF刺激胰腺导管上皮细胞增殖,寻求最佳浓度。结果大鼠胰腺导管上皮细胞表达Nestin和CK19,不表达Insulin及Glucagon;不同浓度KGF刺激胰腺导管上皮细胞2 d后,细胞均有不同程度增殖,20μg/L KGF作用2 d时,细胞数为0.35±0.03,显著高于对照组的0.27±0.02(P<0.01)。结论 KGF在本实验的剂量范围内可促进胰腺导管上皮细胞增殖,20μg/L为促进增殖的最佳浓度。

Objective To investigate the effect of keratinocyte growth factor（KGF） at different concentrations on the proliferation of pancreatic ductal epithelial cells（PDECs）.Methods The PDECs were identified by immunocytochemical stain and RT-PCR.PDECs counting was carried out at different KGF concentrations.Results Nestin and CK19 were positively expressed in PDECs by RT-PCR and immunocytochemical stain;PDECs were stimulated by different concentrations of KGF after 2 days,and PDECs number stimulated by 20 μg/L KGF was 0.35±0.03,much higher than that of the control group which was 0.27±0.02.Conclusions KGF at different concentrations can significantly stimulate the proliferation of PDECs,the optimized concentration found in our tests is 20 μg/L.