齐墩果酸对抗小鼠胰岛细胞糖脂毒性和凋亡促进胰岛素分泌

Oleanolic acid promote insulin secretion against glucolipotoxicity and apoptosis

目的观察齐墩果酸对高糖脂毒环境下胰岛细胞分泌功能与细胞凋亡的影响。方法小鼠原代胰岛细胞培养,不同浓度齐敦果酸干预后放免法检测胰岛素含量,MTT法检测胰岛细胞活力,流式细胞技术检测胰岛细胞凋亡情况。结果高葡萄糖和棕榈酸联合培养能抑制胰岛细胞活力,显著抑制胰岛素分泌,齐墩果酸能对抗该作用。结论齐敦果酸在一定程度上能对抗糖脂毒性,促进胰岛细胞分泌胰岛素,抑制胰岛细胞凋亡。

Objective To observe the effects of Oleanolic acid on islet cell proliferation and apoptosis under high glucose and fat toxicity.Methods Mouse primary islet cell was cultivated under high glucose and fat,then different concentration of Oleanolic acid are administrated for intervention,radioimmunoassay to detect insulin secretion,islet cell proliferative activity was determined by MTT assay;islet cell apoptosis was determined by flow cytometry.Results Islet cell proliferation and insulin secretion were inhibited by the mixed culture medium of glucose and palmitic acid,Oleanolic acid can against it.Conclusions Oleanolic acid can protect islet cell from high glucolipotoxicity,enhance viability of islet cell and inhibit islet cell apoptosis.