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二氯化钴致人结肠癌细胞系CacyBP/SIP核转位 被引量：5

Effect of CoCl_2 on CacyBP/SIP nuclear translocation of colon carcinoma cell lines
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摘要 目的观察和探讨低氧可否引起人结肠癌细胞系CacyBP/SIP核转位。方法用人结肠癌细胞系HCT-116的cDNA序列,用分子克隆法构建过表达CacyBP/SIP的绿荧光慢病毒载体,转染至结肠癌SW480细胞,用CoCl2作为刺激因子,激光共聚焦显微镜和Western blot检测CacyBP/SIP的表达及定位。结果构建了过表达CacyBP/SIP的慢病毒载体,转染至结肠癌细胞,CoCl2刺激前CacyBP/SIP定位和表达主要在细胞胞质,刺激后CacyBP/SIP定位和表达在细胞胞质和胞核。结论 CoCl2刺激后可致CacyBP/SIP核转位。 Objective To observe and evaluate the effect of hypoxia on CacyBP/SIP nuclear translocation of the human colon carcinoma cell lines.Methods Taking cDNA sequence of colon carcinoma cell line HCT-116 cells as the subject,the study mainly builds overexpressed lentiviral vector with Green fluorescent protein CacyBP/SIP by Molecular cloning,and then transfects it into SW480 cells of colon carcinoma.Taking CoCl2 as the Stimulating factor to find the location and expression of the CacyBP/SIP with Laser Scanning Confocal Microscope and Western blot.Results The construction of lentiviral vectors for the overexpression of CacyBP/SIP was transfected into colon cancer cells,and it has been found that before the stimulation of CoCl2,CacyBP/SIP is located and expressed in the cytoplasm,and then in both cytoplasm and nucleus after the stimulation of CoCl2.Conclusions The stimulation of CoCl2 can lead to the nuclear translocation of CacyBP/SIP.
作者 谢福利 仇长青 赵盈盈 杨博 冯珊珊 翟惠虹
机构地区 宁夏师范学院医学院 宁夏医科大学临床学院总医院消化内科
出处 《基础医学与临床》 CSCD 北大核心 2012年第11期1312-1317,共6页 Basic and Clinical Medicine
关键词 CacyBP/SIP CoCl2 核转位 结肠癌 CacyBP/SIP CoCl2 nuclear translocation colon carcinoma
分类号 R735.35 [医药卫生—肿瘤]
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基础医学与临床
2012年 第11期

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