微生物发酵生产β-葡萄糖苷酶

Producing of β glucosidase by Fermentation

分别用土壤分离微生物和现有菌株发酵生产 β-葡萄糖苷酶 ,经酶活力比较 ,筛选酶活较高的产酶菌株。其产酶条件为 :以初始 p H为 5.5的麸皮、 ( NH4) 2 SO4等为发酵培养基 ,在 3 0℃条件下 ,于 1 40 r/min摇床转速下培养 1 2 0 h,获较高酶产量 ,酶活达 9.1 2

The isolated strains from soil and the existing strains were used to produce the β glucosidase The optimum ferment conditions were as follows:the pH of medium which consists of bran and (NH 4) 2SO 4,etc was 5 5,the culture temperature was 30℃ and the shaker rotary rate was 140 r/min Under thoes optimum conditions, the activity of β glucosidase was 9 12U/ml